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Subsequently, a 200-μl fixation buffer was used to repair the cells for 20 min. Right after washing the cells 3 times with precooled PBS, 1× intracellular staining permeabilization wash buffer containing 1% DAPI (blue) was placed on mark the nuclei with the cells. 10 minutes later on, the cells ended up washed thrice with precooled PBS again, an

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