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Subsequently, a 200-μl fixation buffer was used to repair the cells for 20 min. Right after washing the cells 3 times with precooled PBS, 1× intracellular staining permeabilization wash buffer containing 1% DAPI (blue) was placed on mark the nuclei with the cells. 10 minutes later on, the cells ended up washed thrice with precooled PBS again, an
To look through Academia.edu and the wider Online faster plus much more securely, make sure you take a several seconds to up grade your browser.12b has preclinical anti-inflammatory action from the lung with substantial plasma protein binding and reduced bioavailability to reduce systemic Negative effects (De Savi et al., 2014). It is unclear if t
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